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3.5. Determination of the Minimum Fungicide Concentration (MFC)
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After incubation of the 96-well microplates used in the MIC assay, the MFC determination was performed. With the aid of sterile wooden sticks, the mixture from each well (which showed antifungal activity) was transferred to Sabouraud Dextrose Agar plates, which were incubated at 37 °C for 48 h. MFC was defined as the minimum concentration necessary for the absence of colony forming units formation [76].
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