4.7. Thioflavin T Binding Assay

The thioflavin T (ThT) working solution (20 µM) was prepared in TBS and filtered on polyether sulfone 0.2 µm. Just before measurement, 75 µL of ThT working solution was added to 75 µL of protein material (final ThT concentration of 10 µM). ThT fluorescence spectra were recorded with an Agilent Cary Eclipse fluorescence spectrophotometer at ~20 °C in TBS, using a 10 mm optical pathlength quartz GS cell (Hellma), a data pitch of 1.0 nm, and excitation and emission sw of 10 nm.