Transfection of plasmids

MM Mitsuhiro Machitani
JT Junko Takei
MK Mika K. Kaneko
SU Saori Ueki
HO Hirofumi Ohashi
KW Koichi Watashi
YK Yukinari Kato
KM Kenkichi Masutomi
request Request a Protocol
ask Ask a question
Favorite

To overexpress SARS-CoV and -CoV-2 nsp12 transiently, 293T cells were transfected with plasmid vectors carrying a FLAG-tagged nsp12 expression cassette, pCMV-FLAG-CoV-nsp12 and -CoV-2-nsp12 using Lipofectamine 2000 (Thermo Fisher Scientific). After 72 h of incubation, cells were lysed in RIPA buffer (Nacalai Tesque) for western blotting analysis.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A