4.3.1. Sephadex G75

BG Berta Gorospe
JM José J. G. Moura
CG Carlos Gutierrez-Merino
AS Alejandro K. Samhan-Arias
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Samples were centrifuged at 8000× g for 30 min, and the supernatant was taken and loaded onto a Sephadex G75 (2.5 cm d × 50 cm l), previously equilibrated with Tris 150 mM at pH 8. Chromatography was run at a constant flux rate (2 mL/min and eluting fractions were characterized by SDS-PAGE to determine the fractions containing bands corresponding to the molecular weight of Cav-2α. These fractions were combined and dialyzed several times against 5 L of Tris 5 mM at pH 8 and concentrated using a Vivaspin Turbo 15 (3 kDa cut off) (Sartorious; Gloucestershire, UK).

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