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Inhibition of conidial germination assay

AA Amer M. Abdelaziz
MK Mohamed H. Kalaba
AH Amr H. Hashem
MS Mohamed H. Sharaf
MA Mohamed S. Attia
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A suspension of 10 days old F. oxysporum conidia was prepared as previously mentioned in the antifungal assay. In this assay, the effects of endophytic fungi crude extracts on F. oxysporum conidial germination were examined at varieing concentrations (2.5, 5 and 7.5 mg/mL) as performed by Rongai et al. (2012) with some modifications. Each well of the microtiter plate received 200 μL of a combination containing: 80 μL of conidial suspension, 100 μL of double strength PDB, and 20 μL of the tested fungal crude extract. Each treatment was carried out in three wells, with one plate row containing an untreated spore suspension in PDB as a negative control. Conidial germination was determined by mounting 10 μL samples of each treatment on a hemocytometer slide after 24, 48, and 72 h and counting the number of germinated and non-germinated spores in five squares at 200 × magnification. The percentage of germination was determined and averaged for the three wells using the following equation:

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