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Cathepsin B activity was measured from cell lysate using cathepsin B activity kit (Calbiochem, CBA001) according to the supplier’s instruction. Briefly, the lysates were incubated on ice for 30 min and centrifuged at 13,000 rpm for 20 min. Cathepsin B activity was then measured from supernatant using synthetic substrate at an excitation wavelength of 360 nm and an emission wavelength of 460 nm.
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