The probe was positioned by pressing on the center of the testicle transversely, and at least three clips per testicle were measured (technical replicates). The software Ecotext® (HUMECO; Huesca, Spain) was used for analyzing the parenchyma echotexture. The following parameters were measured: Ecotext 1 (EC1: black pixels number), Ecotext 2 (EC2: white pixels number), Ecotext 3 (EC3: mean gray level of pixels), the tubular density (density of tubules/cm2), the tubular diameter [mean diameter (μm) of the lumen of the seminiferous tubules] and the tubular area [proportion (%) of the total area that was occupied by the lumen of the tubules in the parenchyma].
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