2.4. Nile Red and BODIPY staining

Lipid droplet formation and lipid peroxidation were analyzed via Nile Red and BODIPY staining, respectively. Briefly, AML12 cells were washed with 1X PBS, then incubated with 0.1 μg/ml Nile Red (Sigma, 72485) or 1 μM BODIPY 581/591 C11 (Thermo Fisher Scientific, D3861) for 30 min in normal culture medium at 37 °C. Cells were then washed twice with 1X PBS and imaged. For the tissue staining, liver tissue from the indicated groups were fixed in 3.7% paraformaldehyde, rinsed several times with 1X PBS, then 20 μm thick sections were cut using a vibratome (Precisionary Instruments LLC). Tissue sections were then incubated with 1 μg/ml Nile Red or 5 μM BODIPY 581/591 C11 for 30 min in 1X PBS at room temperature and washed twice with 1X PBS. Finally, the tissue sections were mounted with Vectashield (Vector Labs) and imaged.