Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Flow cytometry

QW Qichao Wang
ZL Zhenfu Liu
ST Shuhong Tang
ZW Zhen Wu
request Request a Protocol
ask Ask a question
Favorite

Rats were sacrificed by cervical dislocation. Peripheral blood mononuclear cells (PBMCs) were isolated from fresh heparinized blood samples of rats by density gradient separation using Ficoll density gradient (TBD Science, China). PBMCs were then stained with the PE-anti-CD8, FITC-anti-CD4 (BioLegend, San Diego, USA), and incubated at 4 °C for 30 min (avoid light). Subsequently, cells were washed twice with PBS and re-suspended in a 4% paraformaldehyde solution with a final volume of 300 μL for analysis. The percentage of CD4+ T cells and CD8+ T cells in peripheral blood were detected by flow cytometry and analyzed by FlowJo VX (Treestar, USA).

Copyright and License information: The Author(s) ©2022
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A