Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.5 Infarct volume measurement

LY Lingling Yang
XS Xiaojuan Su
FL Fangfang Lu
RZ Rong Zong
SD Shuqin Ding
JL Jing Liu
GW Gidion Wilson
LL Liuyan Li
YY Youyue Yang
WW Weibiao Wang
XW Xiaoying Wang
JC Jianyu Chen
XM Xueqin Ma
ask Ask a question
Favorite

Cerebral infarct volume was determined by using the 2,3,5-triphenyltetrazolium chloride (TTC) staining method. The rats were anesthetized, the blood samples were obtained from the femoral artery, the brain was removed immediately and cut into five coronal slices averagely, and then the slices were immersed in 2% TTC for 45 min at 37°C in the dark and turned them every 15 min. The results were analyzed by measuring the normal (red) and infarct volume ratio of the brain tissue (white). The cerebral infarction volume ratio was calculated using the following formula: the infarcted volume of the brain/the total volume of the brain × 100%.

Copyright and License information:  ©2022 Yang, Su, Lu, Zong, Ding, Liu, Wilson, Li, Yang, Wang, Wang, Chen and Ma.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A