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2.5. shRNA Transfection

CC Chieh-Yin Chen
YL Yu-Hsuan Li
WL Wan-Ling Liao
MO Muhammet Oner
YC Yu-Chiao Cheng
FL Fang-Ling Liu
PC Pang-Ting Cheng
AC Ayse Celik
JW Jyh-Horng Wu
CL Chih-Ho Lai
JH Jer-Tsong Hsieh
HL Ho Lin
TC Ting-Chieh Chang
CC Chih-Ying Chang
MC Mei-Chih Chen
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To knockdown specific proteins, small hairpin RNAs, shGFP (TRCN0000072192) and shTP53 (TRCN0000003756), were obtained from the Nation RNAi Core Facility of Academia Sinica, Taiwan. The shRNA was diluted in Opti-MEM (Gibco) and premixed with Lipofectamine 3000 and P3000 transfection reagents (invitrogen) according to the manufacturer's instructions. Then the mixture, which is a liposome/nucleic acids complex, was transfected into the LNCaP cells for 48 hours.

Copyright and License information:  ©2022 Chieh-Yin Chen et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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