2.10 Characteristics of immune and transcriptome traits in m6Ascore phenotypes

Spearman’s correlation analysis was performed to quantify the correlation between the m6Ascore and immune infiltration, hallmark gene set, HLA molecules, and immune checkpoints. Based on the median of all m6Ascore, the patients were classified into high and low m6Ascore groups. Kruskal–Wallis test was performed to estimate the enrichment score of immune infiltration, HLA molecules, and immune checkpoints between these two groups. The differentially expressed circRNAs were screened out with a threshold of |log2FC| ≥ 2 and p-value <0.05. The circRNAs-m6A regulators and m6A regulators-m6A related genes interacting pairs were downloaded from the ENCORI database to further analyze the circRNA-mediated regulatory network in m6A related genes (Li et al., 2014).