Cyclosporine administration and sample collection

There was a 24-h interval after pretreatment to reduce the occurrence of antibiotic-drug interactions. After the 24 h interval, a single dose of CSA (100 mg/kg), dissolved in virgin olive oil, was administered to the rats via oral gavage. Whole blood samples were collected at 1, 2, 4, 6, 7, 8, 10, 12, 24, 48 and 72 hours after CSA administration. After the last whole blood sample was collected, the rats were sacrificed with pentobarbital sodium by intraperitoneal injection and dissected to collect tissue and cecum contents. All samples were snap frozen after collected and kept at-80°C until further analysis.