Immunofluorescence microscopy

MJ Myeongwoo Jung
SR Seungyeon Ryu
CK Chongtae Kim
SC Seongho Cha
HK Hoin Kang
EJ Eunbyul Ji
YH Youlim Hong
YL Youngjoon Lee
SH Sukyoung Han
SJ Seung Min Jeong
WK Wook Kim
EL Eun Kyung Lee
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Cells were fixed with 4% FA solutions and paraffin-embedded pancreatic tissues were deparaffinized. Fixed cells and tissues were permeabilized with Triton X-100, sequentially incubated with blocking solution and primary antibodies, including Col18a1 (Invitrogen™), Serpin E1, CD68 (Abcam Plc.), NG2 (Sigma-Aldrich), PECAM-1 (Santa Cruz Biotechnology, Inc.), and MPO (Agilent Technologies, lnc., Santa Clara, CA, USA), at 4 °C overnight, and further incubated with secondary antibodies conjugated with Alexa Flour® 488 or Alexa Flour® 555 (Abcam Plc.). DAPI (4’,6-diamidino-2-phenylindole) solution (Invitrogen™) was used to stain the nuclei. Fluorescence signals were observed and imaged using the ZEISS Axio Imager M1 microscope (Carl Zeiss, Oberkochen, Germany).

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