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Effects of biogenic amine-related drug injection on behavior

TW Tomohiro Watanabe
KS Ken Sasaki
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Two µl of dopamine (Sigma-Aldrich) and octopamine (Sigma-Aldrich) solution (10–4, 10–3, and 10–2 M) were dissolved in 0.1 M phosphate buffer (pH 7.0) or 2 µl of 0.1 M phosphate buffer (control). Epinastine (TCI, Tokyo, Japan, octopamine receptor antagonist44,45) was dissolved in 0.1 M phosphate buffer to the concentration of 10–2 M. To examine the roles of biogenic amines in the locomotion, light preference, and flight behaviors of bumble bee males, prepared solutions were injected into the abdomens of 4 day-old males using a 10-µL microsyringe with a fine needle. The duration of acclimatization was 15 min after drug injection before observing locomotor activities.

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