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Lentivirus packaging and transduction

ZZ Zhong Zheng
PX Peng-Peng Xu
LW Li Wang
HZ Hui-Jin Zhao
XW Xiang-Qin Weng
HZ Hui-Juan Zhong
BQ Bin Qu
JX Jie Xiong
YZ Yan Zhao
XW Xue-Feng Wang
AJ Anne Janin
WZ Wei-Li Zhao
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To overexpress miR21 in A20 cells, purified plasmids pGMLV-miR21 or control vector were transfected into HEK-293 T cells with package vectors using lipofectamine 2000. The supernatant of HEK-293 T cell culture was then condensed to a viral concentration of approximately 3 × 108 transducing units/ml. The lentiviral particles were incubated with A20 cells for 8 h. The stably transduced cells were selected by green fluorescence protein.

Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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