Gene structure and protein structure analysis

SZ Shuo Zhang
XH Xuewei Hu
JD Jiejing Dong
MD Mengxiang Du
JS Juqi Song
SX Shangyuan Xu
CZ Changjiang Zhao
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Gene structure analysis of the OsBSK family was performed using TBtools. The motif of the OsBSK family protein was analyzed by MEME (http://meme-suite.org/tools/meme), and the protein secondary structure was predicted by prabi (https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_sopma.html) [41], and the protein tertiary structure was predicted by SwissModel (http://swissmodel.expasy.org/) [42]. In addition, the promoter sequence 2000 bp upstream of the gene translation initiation site was extracted and submitted to Plant CARE (http://bioinformatics.psb.ugent.be/webtools/plantcare/html/) [43] for cis-element analysis and used TBtools to make a statistical map of the number of cis-elements.

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