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The workflow of this study is illustrated in Supplementary Fig. S1 and S2. WGS was performed in tumor and matched “normal” germline (blood) DNA. BAM files were preprocessed following GATK best practices and sequence reads were mapped to GRCh37 using Burrows-Wheeler Aligner. Resulting CRAM files were then used for variant calling using Sentieon's genomics package (bioRxiv 115717v2). The mean sequencing coverage was 32X for normal samples and 88X for tumor samples.
Copyright and License information: ©2022 The Authors; Published by the American Association for Cancer Research
This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.
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