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Cells were cultured in RPMI-1640 supplemented with 5% human serum, 2mM L-glutamine and 10μg/ml penicillin-streptomycin (Gibco). rhIL-2 was used for T cell culture and expansion. Violet Proliferation Dye 450 (VPD) (1μM, BD Bioscience), Brefeldin A (10μg/ml, Sigma-Aldrich) and 4% paraformaldehyde (Sigma-Aldrich) were used. POM-1 and AB-680 (MedChemExpress) drugs, which inhibit CD39 and CD73, respectively, were used as indicated in the figure legends.
Copyright and License information: ©2022 Jotereau, Alameddine, Teusan, Pédron, Jouand, Altare and Godefroy
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