2.4. Molecular analysis of MβL encoding genes

The imipenem-resistant isolates of P. aeruginosa were selected. Next, their total DNA was extracted by the boiling method [13,14] and stored at -20°C until PCR amplification. Polymerase chain reaction (PCR) was carried out to detect blaIMP and blaVIM genes and common subtypes of bla_IMP-1, bla_IMP-2, bla_VIM-1, and bla_VIM-2 with specific primers (Table 1). The gels were stained with KBC stain (CinnaGen, Iran), and the PCR products were visualized with UV light [10].

primers sequence of target genes