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Co-immunoprecipitation (Co-IP) assay was performed using the Pierce Co-IP Kit (Thermo Fisher Scientific, MA, USA) following the manufacturer’s instructions. Cell lysates were prepared and incubated with AminoLink Plus Coupling Resin immobilized primary antibody overnight at 4 °C. Then, the samples were washed three times with 200 μL Wash Buffer and eluted with Elution Buffer for 5 min. The eluates were finally analyzed by western blot.
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