A ChromInst™ (EK100100724, NICSInst™ Library Preparation Kit, Xukang Medical Technology (suzhou) Co., Ltd, China) was used to conduct whole-genome amplification (WGA). Quality control of the NGS libraries was performed using Qubit 3.0 and 1.5% agarose gel electrophoresis. Sequencing was conducted using the Illumina platform, HiSeq 2500 (Illumina, San Diego, CA, USA), yielding ∼2 million sequencing reads (single-end, 55 bp) on each sample [19, 20, 25–27]. We sequenced the amplified genome of each sample at the depth × 0.036. The details of WGA and sequencing were described previously [19].
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