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Adjuvant arthritis induction and treatment

ZZ Zhuoyan Zai
YX Yayun Xu
XQ Xuewen Qian
ZL Zihan Li
ZO Ziyao Ou
TZ Tao Zhang
LW Longfei Wang
YL Yian Ling
XP Xiaoqing Peng
YZ Yihao Zhang
FC Feihu Chen
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Rats were ovariectomized or removed adipose tissue around the ovaries before molding. The OVX and sham-operated rats received intradermal immunization into the left hind metatarsal footpad with 0.1 mL heat-killed mycobacteria (10 mg/mL) suspended in Complete Freund’s adjuvant (CFA, Chondrex Inc., USA). On the 20th day after immunization, the rats were randomly allocated for in vivo experiments (n = 6): the normal group, the model group, the OVX and estradiol (E2)-injected group (E2 was injected at 0.1, 0.2, and 0.3 mg/kg) (Sigma, USA, once every 3 days, total eight times), OVX and triamcinolone acetonide (TA)-treated group (positive control, 1 mg/kg, Selleck, USA, intra-articular injection, once every 3 days, total eight times), and the sham group.

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