2.10. Statistical analysis

In our studies, power analysis was conducted assuming a 95% confidence level (p < 0.05), a standard deviation that is 20% of the mean value, a difference in means between groups that is less than or equal to 20% of mean values and statistical power > 80%. In most of our studies, a sample size of 6 animals/group were included. Data are reported as mean ± SEM. A two‐way ANOVA followed by Bonferroni's post hoc test was used to compare the changes in body weight and fecal pellet output following age development, the concentration and frequency responses of tissue isometric force recording, and frequency responses of amperometry detection of NO release, in different groups, 5xFAD vs WT. Unpaired Student t‐tests were used for comparing two groups, such as CMMCs and IJP measurements in 5xFAD vs WT mice. Data were graphed and analyzed using GraphPad Prism 6.0 software. A p‐value of <0.05 was considered statistically significant.