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RNA was harvested with TRI reagent according to the manufacturer’s protocol. Purified RNA was treated with RQ1 DNase (Promega) and cDNA was synthesized with murine leukemia virus (MuLV) reverse transcriptase (New England Biolabs) with oligo(dT)20 priming. All real-time PCR mixes performed with iTaq Universal SYBR green Supermix (Bio-Rad) and analyzed with QuantStudio (v1.5.2). Gene-specific primer sequences can be found in Supplementary Table 1.
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