Blood samples were collected and frozen on the same day as the PET scan. Plasma p-tau181 concentration was measured using in-house Single molecule array (Simoa) methods on Simoa HD-X instruments (Quanterix, Billerica, MA, USA) at the University of Gothenburg. Methods are described in the Supplementary Information and detailed elsewhere [28, 29]. No blood samples were acquired for 1 participant and 1 sample was excluded due to being >3 SD outside the cohort mean, leaving 73 p-tau181 samples for analysis. To dichotomise the blood plasma p-tau into T−/T+ groups, a cut-off of 17.7 pg/ml was used [30].
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