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High-resolution mass spectrometry for synthesized compounds

KY Koji Yamaguchi
HM Hajime Miyaguchi
YO Youkichi Ohno
YK Yoshimasa Kanawaku
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High-resolution mass spectrometry (HR-MS) measurements for synthesized compounds were performed using a Q Exactive mass spectrometer (Thermo Fisher Scientific, Waltham, MA) with an electrospray ionization (ESI) probe. Full scan mass spectra were recorded in positive mode. The conditions for ESI were as follows: sheath gas flow rate, 5 units; capillary temperature, 320 °C; spray voltage, 3.5 kV; probe heater temperature, 350 °C; S-lens RF level, 50 units. Products were dissolved in a 1:1 (v/v) mixture of water–acetonitrile containing 0.1% formic acid at a concentration of 1 μg/mL and infused into the spectrometer at a flow rate of 10 μL/min.

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