2.5.3. Confocal laser scanning microscopy study

A longitudinal section of the skin tissue was examined and visualized using a microscope (LSM 710; Carl Zeiss, Oberkochen, Germany). The excitation wavelength of the FDA is 488 nm, while the emission wavelength is 530 nm. The investigation provided qualitative and quantitative evidence of the system permeation pattern considering skin thickness. Scanning occurred optically from the surface of 0 μm to 300 μm at 15 μm increments. Permeation of the foam system in skin layers was reflected by structuring a 3D plot using Z-stack mode (Abdellatif et al., 2017).