The intraperitoneal glucose tolerance test (IPGTT) was performed on overnight-fasted rats to evaluate glucose tolerance at the end of the study. Rats were fasted for 8 h and then given a glucose load (1.5 mg/kg body weight, intraperitoneal), and blood samples were collected from the tail vein at 0, 15, 30, 60, 90, and 120 min after the glucose was given. The glucose levels were determined using a CardioChek™ analyzer (Polymer Technology Systems, Inc., IN, USA).
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