Quantitation of mouse cytokines in serum obtained from mice in the final short term toxicity study described above was accomplished using the LEGENDplex™ Mouse Anti-Virus Response Panel (BioLegend) and the associated LEGENDplex™v8 Data Analysis Software. Each serum sample was diluted 1:1 and then assessed in two wells. The assay and acquiring of the flow samples on a Beckman Coulter CytoFLEX flow cytometer followed the detailed instructions provided by the manufacturer’s LEGENDplex™ manual.
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