Analysis of inflammatory cytokines using LEGENDplex

LO Lyra B. Olson
NH Nicole I. Hunter
RR Rachel E. Rempel
HY Haixiang Yu
DS Diane M. Spencer
CS Cynthia Z. Sullenger
WG William S. Greene
AV Anastasia K. Varanko
SE Seyed A. Eghtesadi
AC Ashutosh Chilkoti
DP David S. Pisetsky
JE Jeffrey I. Everitt
BS Bruce A. Sullenger
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Quantitation of mouse cytokines in serum obtained from mice in the final short term toxicity study described above was accomplished using the LEGENDplex™ Mouse Anti-Virus Response Panel (BioLegend) and the associated LEGENDplex™v8 Data Analysis Software. Each serum sample was diluted 1:1 and then assessed in two wells. The assay and acquiring of the flow samples on a Beckman Coulter CytoFLEX flow cytometer followed the detailed instructions provided by the manufacturer’s LEGENDplex™ manual.

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