4.5. Chromosome Location and Synteny Analysis

According to the GFF3 file of the reference genome, RrOSC gene location was illustrated by the Gene location visualize function of the GTF/GFF tool of TBtools toolkit 1.086 [33].

Inter-species synteny analysis was conducted by reciprocal BLASTP search for potential homologous gene pairs (E < 10 − 5, top five matches) of the whole genome, and the syntenic regions were predicted by the MCScanX tool of TBtools toolkit 1.086 [34]. The gene duplication events were predicted according to syntenic regions by the ‘duplicate_gene_classifier’ program of MCScanX [34].