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4.3. Cell Viability Assay after Incubation with Quercetin and Fingolimod

AM Albena Momchilova
GN Georgi Nikolaev
SP Stefan Pankov
EV Evgenia Vassileva
NK Nikolai Krastev
BR Bozhil Robev
DK Dimo Krastev
AP Adriana Pinkas
RP Roumen Pankov
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After the incubations, cell viability was determined by tetrazolium salt measurement (MTT assay), involving assessment of succinate dehydrogenase-induced conversion of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide into formazan crystals. Formation of formazan was measured at 570 nm. The viability of the cells after incubations was estimated as percentage of the absorbance of the treated cells compared to controls.

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