4.6. Fluorescence-Activated Cell Sorting (FACS)

Trypsinized cells were incubated with a mixture of equimolar ratio of recombinant proteins as indicated, for 30 min at 4 °C in FACS Buffer (PBS with 0.5% BSA and 2 mM EDTA). Cells were washed twice in FACS Buffer and analyzed on an Accuri™ C6 Plus flow cytometer (BD Bioscience, San Jose, CA, USA). The relative mean fluorescence intensities were normalized and plotted against the concentration of the nano-HER2 at monomer concentration. The data shown here are single-point measurements.