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2.6.4. Trypan Blue Dye Viability Exclusion Assay for A375 CSC Post-Irradiation
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The percentage of viable cells in the categorized cell suspensions were determined using the trypan blue dye viability exclusion assay. An automated cell counter was used to count cell suspensions containing equal parts of 0.4% (w/v) trypan blue dye (Invitrogen, Trypan Blue Stain (0.4%), Thermo Fisher-T10282, Waltham, MA, USA).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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