2.1.3 Foliar application of CFS

For the foliar application of cell-free supernatant, a bacterial extract material was prepared in from cultures of Devosia sp. strain (SL43), isolated from Amphicarpaea bracteate plants growing wild along the shore of Lac St. Louis, Sainte-Anne-de-Bellevue, Quebec, Canada. Three dilutions were prepared from the extract material, and selected for foliar spray doses on canola and soybean based on our seed germination experiments with the extracted material (Shah et al., 2022); minimum (1:100), intermediate (1:500) and maximum (1:1000) dilutions. ( Table 2 ). CFS was sprayed on canola and soybean at the 4-5 leaf stage and 3-4 trifoliate stage, respectively. The salt stress levels, growth conditions and experimental design for both crops were the same as used in the previous experiment.

Treatments – Foliar application of CFS on canola and soybean.