3.3.2. Minimum Bactericidal Concentration (MBC)

The microtitre broth dilution method was also chosen for MBC determination. It was the lowest compound concentration, killing most (99.9%) of the bacterial inocula after 24 h incubation at 37 °C. The calculation of MBC was carried out using the Ozturk and Ercisli method [46]. It was performed on all conjugates tested. Under aseptic conditions, we took ten microliters from the MIC experiment well (MIC value) and two wells above the MIC value well and spread them on MHA plates. After 18–24 h of incubation at 37 °C, the colony count was determined. The MBC value was influenced by the concentration of sample that produced ten colonies. Each experiment was performed in triplicate.