Preparation of cigarette extract

ML Min-yan Li
YQ Yan-qin Qin
YT Yan-ge Tian
KL Kang-chen Li
BO Brian G. Oliver
XL Xue-fang Liu
PZ Peng Zhao
JL Jian-sheng Li
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The mainstream cigarette smoke was sucked into a 50 mL syringe, and then slowly injected into the serum-free Dulbecco’s modified eagle medium (DMEM). The optical density at 320 nm wavelength was measured with a spectrophotometer, and adjusted to 1.8–2.0 with DMEM. Subsequently, the prepared CSE solution was filtered (0.22 μM) to obtain a 100% CSE solution.

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