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Mean arterial pressure (MAP) monitoring
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Tail cuff blood pressure system (IITC Life Science Inc.) was used for the measurement of MAP. First, the rats were sufficiently acclimated to the restraint holder, and each rat was separated by an opaque partition. Then the tail was warmed with a warming pad for 15–20 min (until the rat was no longer irritable) before each cycle of blood pressure measurements. The final data was obtained from the average of 10 valid data. The data of animals those could not cooperate well in the experiment were eliminated.
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