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In vivo analysis of heart function (tdtk)
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R94C02::tdTomato (attP2) (Klassen et al., 2017) was combined with Hand-Gal4 as stable stock. Flies were then crossed to RNAi lines and reared to 3 weeks of age. To assay fluorescent hearts in vivo flies were mounted as described (Klassen et al., 2017) and were illuminated with green light (3 mW power). Five s movies of the beating heart were acquired at 270 fps and high-speed recordings were then processed using a custom R script (Vogler, 2022a).
Copyright and License information: ©2022, Saha, Spinelli et al
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