QRT-PCR validation was performed to confirm the results of the expression levels of miRNA after transfection. Total RNA was extracted from LX-2 cells and THP-1 cells using TRIzol reagent (Invitrogen, Inc., Waltham, MA, USA). Then the RNA was applied for reverse transcription and quantitative real-time PCR using TaqMan Small RNA Assay kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s user guide on the ABI 7500 real time PCR system (Applied Biosystems, Foster City, CA). The fold changes of genes were calculated using 2−ΔΔCt method. The miRNA expression was normalized to RNU6B. All primers were purchased from Applied Biosystems (Foster City, CA). More details are provided in the Additional file 1.
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