MCF7 and T47D cells were maintained in the phenol-red-free media supplemented with 5% charcoal/dextran-treated FBS. The resistant cell lines were generated by long-term treatment with 1 μM tamoxifen or fulvestrant (MedChem Express, United States) as previously reported (Thrane et al., 2015; Zhu et al., 2018).
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