2.4.2. Trolox Equivalent Antioxidant Capacity (TEAC) Assay

The assay was carried out using the ABTS⁺ method as described by Re et al. [28] with some modifications. Briefly, the ABTS radical cation was generated by mixing 10 mL of 2 mM ABTS with 100 µL of potassium persulfate (2.45 mM). After the reaction mixture is kept in the dark at room temperature for 16 h, methanol is used to dilute the ABTS solution to obtain an absorbance of 0.700 at 734 nm. Two hundred µL of the essential (1 mg/mL) was mixed with 2 mL of diluted ABTS solution. The mixture was allowed to react for 1 min, and the absorbance was measured at 734 nm. Trolox is used as a reference standard. The results were expressed as an IC₅₀ value (µg/mL).