To determine the functional groups from the reducing agent attached to nanoparticles, a freshly prepared sample of CuONPs was placed on a glass slide and allowed to dry at room temperature. Afterward, the Fourier transform infrared spectroscopy (FTIR) spectrum was collected using a Bruker Tensor 27 FT-IR spectrometer (SpectraLab Scientific Inc., Markham, ON, Canada) with a resolution of 0.5 cm−1 in transmission mode. An average of 5 scans for the spectrum was recorded over the 500–4000 cm−1 wavelength range.
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