2.10. Swimming Motility Assay

Motility activity was evaluated in LB broth containing 0.3% agar concentrations as previously described [18]. Both SCFAs were added to LB broth at a final concentration of 0 mg/mL, 0.25 mg/mL, 0.5 mg/mL, 1 mg/mL, and 2 mg/mL, then the plates were placed at room temperature for 40 min. The semi-solid agar plates were spotted with 2 μL volumes of S. Typhimurium SL1344 (10⁶ CFU/mL) at the center of the plates and incubated at 37 °C for 12 h or 24 h, respectively. Medium without SCFAs was used as a control.