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Cytotoxicity studies

DZ Dongya Zhang
ST Sidan Tian
YL Yanjie Liu
MZ Meng Zheng
XY Xiangliang Yang
YZ Yan Zou
BS Bingyang Shi
LL Liang Luo
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U87MG or CSC2 cells were inoculated into a 96-wells plate (1 × 104 per well) with cells attaching to the wall for 24 h. ARNGs@TMZ/ICG, ARNGs@TMZ, ARNGs@ICG, free TMZ, free ICG (TMZ: 10 μg mL−1, ICG: 10 μg mL−1) and blank ARNGs were then incubated with U87MG cells, respectively. after 4 h of incubation, NIR laser (808 nm, 0.5 W cm−2) was applied on corresponding cells for 5 min, and the cells were incubated for another 44 h. Viability of the cells was analyzed using the CellTiter-Lumi™ luminescent cell viability assay kit.

The inhibitory concentration (IC50) of ARNGs@TMZ/ICG was carried out in U87MG cells similarly using a series drug concentration (0.125, 2.5, 5, 10, 20, 40 and 80 μg mL−1). CI values were calculated using the Chou-Talalay method with CompuSyn software.

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