2.1. Cell culture

C2C12-normal mouse myoblasts (American Type Culture Collection, ATCC®, LGC Standards Sp. z o.o., Poland) were used in the study. C2C12 cells were grown in DMEM (Sigma-Aldrich, Poznan, Poland), low glucose (1 g/L), and GlutaMAX™ Supplement (Gibco). The culture medium was supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), and penicillin/streptomycin (100 U/mL, Gibco). Cells were passaged twice a week. The cells were grown in an incubator at 37 °C with 5% CO₂. Cells were tested routinely for mycoplasma once per month.