Quantification and statistical analysis

Data were presented as mean ± standard error of the mean (SEM) and analyzed using MATLAB (MathWorks) and Prism8 (GraphPad Software). Firing rates were smoothed with a Gaussian kernel (σ = 10 ms). The mean baseline firing rate of each neuron was calculated during 1000 ms before the environment onset. The statistical significances of the neuronal and behavioral discriminations were tested using one-way analysis of variance (ANOVA) and two-way repeated measures ANOVA with Tukey posthoc test. The correlations between neuronal and behavioral responses were analyzed by Pearson correlation analysis.