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2.8 Evaluation of adipose tissue derived stem cell viability
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Calcein AM (5 μg/ml; Thermo Fisher, Australia) and propidium iodide (PI, 1 μg/ml; Thermo Fisher, Australia) were used for evaluation of ADSC viability as per the manufacturer’s manual. Samples were incubated with the assay for 30 min in an incubator (37°C with humidified 5% CO2) and washed with fresh CM before imaging. AxioImager microscope (Zeiss, Germany) was used for imaging and analysis.
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