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For experiments with effector cells (NTD T cells, CD19-CAR, or CD6-CAR-T cells), these were cocultured with target cells for 24 h at a defined E:T ratio, and the supernatant was assessed for IFN-γ secretion levels using enzyme-linked immunosorbent assay kits (Thermo Fisher Scientific, USA) according to the manufacturer's instructions.
Copyright and License information: ©2022 Published by Elsevier Inc.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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