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Secondary pruning of contours

LZ Louis Zhang
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As illustrated in Fig. 2, the sure foreground produced by the local minima detector contains both accurately detected colony centers and a significant number of artifacts. To differentiate between the two groups, the resulting contours generated from the watershed are filtered based on their mean HSV hue, circularity, inertia, and area, using values derived from the previously determined single colony cluster(s).

Overview of local minima detector. a A binarized inverted mask of a grayscale plate image containing only those regions that have been deemed as noise/colony clusters; b The local extrema detected. Note the high amount of noise from the plate borders that will need to be pruned through a comparison with the ground truth

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